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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke

Fig. 7

Inactivation of STAT3, partly, inhibits the pro-angiogenic effect of iMSC-sEV after the experimental stroke model. a, b Rats were intravenously treated with vehicle (500 μl, PBS), iMSC-sEV (1 × 1011 particles in 500 μL PBS), or iMSC-sEV with stattic (3.75 mg/kg) 4 h after MCAO; angiogenic ability was assessed 7 days after MCAO or Sham surgery. a Representative images of immunofluorescence staining of CD34 in the peri-infarct area. Scale bar = 100 μm. b Quantification analysis of CD34+ cells normalized to that in sham group. N = 3 per group. c–f HUVECs were challenged by OGD for 8 h, followed by treatment with vehicle (PBS), iMSC-sEV (1 × 109 particles/mL), or iMSC-sEV with stattic (5 μM) in normal culture medium for another 24 h. HUVECs cultured under normoxia condition without any treatment were deemed as control. c Representative images of crystal violet staining of HUVECs in the transwell assay. Scale bar = 25 μm. d Quantification analysis of relative migration rate. N = 3 per group. e Representative images of the tube formation assay. Scale bar = 5 μm. f Quantification analysis of relative tube length in the indicated groups. N = 3 per group. Data are presented as mean ± SD. *P < 0.05

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