Fig. 8
Activation of COX2/PGE2 pathway abolished the protection of EPC-EXsmiR-137 against apoptosis and mitochondrial dysfunction. a Representative bands of PGE2 at different concentrations and GAPDH measured by western blot. b Quantification of PGE2 expression at different concentrations, the densities of the protein bands were normalized to GAPDH, n = 4. c Representative bands of Cyt-C, cleaved caspase-3, and β-actin proteins by western blot in each group, β-actin served as the reference protein. d Quantification of relative expression of Cyt-C in each group, n = 4. e Quantification of relative expression of cleaved caspase-3 in each group, n = 4. f Representative Annexin V/PE staining result measured by flow cytometry. g Summarized data of the apoptosis rate in each group, n = 4. h Representative DHE staining result measured by flow cytometry. i Summarized data of the relative ROS level in each group, n = 4. j Quantification of ATP content in the SH-SY5Y cells of each group, n = 6. k Quantification of MMP level in the SH-SY5Y cells of each group, n = 6. *P < 0.05 and **P < 0.01 vs. control group, *P < 0.01 and **P < 0.01