Fig. 5From: Mesenchymal stem cells inhibited the differentiation of MDSCs via COX2/PGE2 in experimental sialadenitisUC-MSCs inhibited the differentiation and enhanced the suppressive activity of PMN-MDSCs and M-MDSCs by COX2/PGE2 and IFN-β, respectively. UC-MSCs co-cultured with bone marrow cells in RPMI-1640 with 40 ng/ml IL-6 and GM-CSF for 4 days by Transwell assays. a qPCR results of MDSC differentiation-related genes in UC-MSCs. b Bone marrow cells were cultured in MSC-CM with COX2 inhibitor or anti-TGF-β1 or anti-IFN-β antibodies for 4 days. And the percentage of MDSCs, PMN-MDSCs, and M-MDSCs was detected by flow cytometry. c, d Bone marrow cells were cultured with PGE2 or IFN-β for 4 days. The percentage of MDSCs, PMN-MDSCs, and M-MDSCs was detected by flow cytometry. e, f MDSC suppressive activity-related genes Arg-1, gp91phox, iNOS, TGF-β1, and IL-1β were detected by qPCR. Error bars indicate SEM. Error bars indicate SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n = 4Back to article page