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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Time-dependent LPS exposure commands MSC immunoplasticity through TLR4 activation leading to opposite therapeutic outcome in EAE

Fig. 4

MSCs-TLR4KO has a limited capacity to inhibit pro-inflammatory Th1 and Th17 cells in vitro. Freshly isolated Naïve T-CD4 cells were differentiated into Th17, Th1, and Treg cells and co-cultured or not with MSCs WT or MSCs-TLR4KO. a Representative dot plot of IL17+, b IFNγ+ and c CD25+Foxp3+ cells on T CD4 cells prompted to in vitro differentiation. Quantification of d IL17- and e IFNγ-producing cells by FACS analysis. f Quantification of CD25+Foxp3+ positive cells. *p < 0.05, **p < 0.01, ***p < 0.001 (control vs. MSCs). Statistical analysis was performed by one-way ANOVA, Kruskal-Wallis ad hoc post test

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Stem Cell Research & Therapy

ISSN: 1757-6512