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Table 2 The sequence of primer used for RT-PCR in this study

From: MicroRNA-503-3p affects osteogenic differentiation of human adipose-derived stem cells by regulation of Wnt2 and Wnt7b under cyclic strain

Gene

Accession No.

5′-3′

Tm (°C)

RUNX2

NM_001015051

F: TAGATAGTGATTGCGTTTGGCTATG

60

R: CACTAAGAAATGTTTCAAGGGTCC

60

ALP

NM_003064

F: GAAAGTCCTTCAAAGCTGGAGTCT

60

R: TCTGGCACTCAGGTTTCTTGTATC

60

SPARC

NM_001309443

F: TGTGATCTAAATCCACTCCTTCCA

60

R: ACAAACCATCCAAACATTTTAAACA

60

Wnt2

NM_004185

F: GGGGCACGAGTGATCTGTG

62

R: GCATGATGTCTGGGTAACGCT

62

Wnt7b

NM_058238

F: CACAGAAACTTTCGCAAGTGG

60

R: GTACTGGCACTCGTTGATGC

60

β-catenin

XM_024453360

F: TTGAACTGTTTGAGGCGAAGAG

60

R: ACTGAACACCGAGTTAGAGGAAT

60

GAPDH

NM_001256799.2

F: GAACGGGAAGCTCACTGG

60

R: GCCTGCTTCACCACCTTCT

60