Fig. 1From: Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkeyGeneration and characterization of knockout iPSCs and expression of genetic alleles of major histocompatibility complex I and II in different iPS cell lines. a DNA sequencing results of different cell lines showed the double peak phenomenon and b T-vector test showed the deletion loci. c Real-time PCR demonstrated B2M knocked out in B2M−/−-iPSCs and B2M−/− and CIITA−/−-iPSCs as well as in their derivatives. CIITA was knocked out in CIITA−/−-iPSCs and B2M−/− and CIITA−/−-iPSCs as well as in their derivatives. d Western blot analysis showed B2M and CIITA expression in different iPS cell lines cultured in stem cell and lymphocyte medium, respectively. e PCR detection of alleles of the major histocompatibility complex I and II in peripheral blood mononucleated cells, WT-iPSCs, B2M−/−-iPSCs, HLA-A−/−-iPSCs, CIITA−/−-iPSCs, and B2M−/− and CIITA−/−-iPSCs. Data are representative of three repeatsBack to article page