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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey

Fig. 1

Generation and characterization of knockout iPSCs and expression of genetic alleles of major histocompatibility complex I and II in different iPS cell lines. a DNA sequencing results of different cell lines showed the double peak phenomenon and b T-vector test showed the deletion loci. c Real-time PCR demonstrated B2M knocked out in B2M−/−-iPSCs and B2M−/− and CIITA−/−-iPSCs as well as in their derivatives. CIITA was knocked out in CIITA−/−-iPSCs and B2M−/− and CIITA−/−-iPSCs as well as in their derivatives. d Western blot analysis showed B2M and CIITA expression in different iPS cell lines cultured in stem cell and lymphocyte medium, respectively. e PCR detection of alleles of the major histocompatibility complex I and II in peripheral blood mononucleated cells, WT-iPSCs, B2M−/−-iPSCs, HLA-A−/−-iPSCs, CIITA−/−-iPSCs, and B2M−/− and CIITA−/−-iPSCs. Data are representative of three repeats

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