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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey

Fig. 5

Teratoma formation, in vivo survival, effects on infiltration of immune cells, and wound healing promotion of knock-out iPS cell lines. a Teratoma generation rates of human WT-iPSCs, B2M−/−-iPSCs, CIITA−/−-iPSCs, and B2M−/− and CIITA−/−-iPSCs in monkeys. b, c Gray scale statistics and images of Western blotting show the presence of CD3, CD20, CD56, and CD68 proteins in the tissue 3 days after Matrigel-embedded human iPSCs were subcutaneously injected into the monkey. d Relative expression levels of human-specific HSP90 sequence demonstrate the survival of transplanted human iPSCs in monkey wound lesions. e, f Gray scale statistics and images of Western blotting show the presence of CD3, CD20, CD56, and CD68 proteins in monkey wound lesions 12 days after human iPSCs were topically disseminated onto the surface of fresh lesions. g Wound closure was measured for 12 days and percentages of wound closure are presented. Data represent the means ± SD. h Immune response of different cell lines to monkey serum measured by ELISA. Data represent the means ± SD; n = 7 in all measurements. Data in a was evaluated by chi-square test. All other data sets were evaluated by paired Student’s t test

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