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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation

Fig. 1

Differentiation and characterization of mouse trophoblast cells. a Representative bright-field images of undifferentiated and differentiated trophoblast cells. b Immunofluorescence imaging of TSCs and TGCs stained with alpha-tubulin. Cells were fixed with paraformaldehyde and stained with alpha-tubulin and Alexa Flour 488-conjugated secondary antibodies. DAPI was used for nuclear (DNA) staining (blue). c Immunofluorescence imaging of TSCs and TGCs for actin. Cells were fixed with paraformaldehyde and stained with Texas red phalloidin and DAPI. d Induction of polyploidy in differentiated TGCs. TSCs and TGCs were fixed, stained with propidium iodide, and analyzed through FACS. e mRNA expression analysis of TSC and TGC specific markers before and after differentiation. Expression levels of TGC-specific markers, Pl-1 and Tpbpa (left), and TSC-specific markers Err1 and Fgfr2 (right) were analyzed through real-time PCR. Error bars represent SEM for 3 independent biological replicates.

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Stem Cell Research & Therapy

ISSN: 1757-6512