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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Transcriptomic analysis reveals differential gene expression, alternative splicing, and novel exons during mouse trophoblast stem cell differentiation

Fig. 3

Analysis of downregulated CPC complex genes in TSCs and TGCs. a mRNA expression analysis of 5 selected downregulated mitotic genes using real-time PCR. Error bars represent SEM of 3 independent biological replicates. b Western blot analysis of Aurora A and Borealin in TSCs and TGCs differentiated for 4 days. c Immunofluorescence imaging of solvent control-treated TSCs, TSCs treated with Aurora A inhibitor MLN8237, and TGCs for actin and nucleus. Cells were fixed with paraformaldehyde and stained with phalloidin Alexa Fluor 488 and DAPI. d FACS analysis of TSCs, TSCs treated with Aurora A inhibitor MLN8237, and TGCs. e mRNA expression analysis of Fgfr2, Pl-1, and Tpbpa in TSCs, TGCs, and TSCs treated with MLN8237 for 3 days. Error bars represent SEM of 3 independent biological replicates.

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Stem Cell Research & Therapy

ISSN: 1757-6512