Fig. 2

Preconditioning enhanced antioxidant response. HC016 cells and hASCs were exposed to 0.25 mM H₂O₂ for 1 h in FBS-free media and lysed just after the insult. a Expression and b quantification of nuclear Nrf2. c Expression and d quantification of HO-1, SOD-1, GPx1, and CAT. Values were normalized to their corresponding loading control. At least three different independent experiments were performed. Data were normalized to control hASCs (dotted line) and expressed as mean ± SD. *p < 0.05, compared with control hASCs, ^#p < 0.05