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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation

Fig. 4

Mitochondrial respiration parameters. Seahorse XF Cell Mito Stress Test was performed in HC016 cells and hASCs under control conditions or after 0.25 mM H2O2 exposure. a Basal respiration. b Maximal respiration. c ATP-linked respiration. d Coupling efficiency. Mitochondrial morphology and MMP in HC016 cells and hASCs. e Confocal microscopy images of HC016 cells and hASCs labeled live with MitoTracker Red CMXRos (red, mitochondria). f MMP was quantified in a plate reader and normalized to cell number. At least three different independent experiments were performed, and results were expressed as mean ± SD. *p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512