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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Hypoxia alleviates dexamethasone-induced inhibition of angiogenesis in cocultures of HUVECs and rBMSCs via HIF-1α

Fig. 5

Hypoxia rescued HUVEC angiogenesis that was inhibited by dexamethasone. a CoCl2 was used to simulate hypoxia. The Matrigel tube assay was used to test the angiogenic ability of HUVECs treated with CoCl2 at 50, 100, 150, 200, or 250 μM in the presence of dexamethasone. b, c The streaked plate assay was used to test the migration ability of HUVECs. The medium contained CoCl2 at 50, 100, 150, 200, or 250 μM in the presence of dexamethasone. The dotted lines highlight the linear scratch/wound for each group of cells. d Cell viability at 24 or 48 h was measured by the CCK-8 assay. e Real-time PCR was used to detect the expression of angiogenesis-related genes in HUVECs treated with 50 μM CoCl2 in the presence of dexamethasone for 72 h. *p < 0.05, compared with HUVECs treated with dexamethasone. n = 3

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