Fig. 6
Hypoxia restored HUVEC angiogenesis via HIF-1α. a Western blotting was used to detect the expression of angiogenesis-related proteins in HUVECs treated with 50 μM CoCl2 for 72 h, and protein expression was quantified by ImageJ software. b HIF-1α gene expression was effectively silenced by specific siRNA sequences. c A Matrigel tube assay was used to detect the angiogenic capacity of HUVECs with HIF-1α silencing. d After HUVEC HIF-1α was silenced, the protein expression of angiogenic signaling molecules at 72 h was determined by Western blotting. *p < 0.05, compared with HUVECs treated with CoCl2. #p < 0.05, compared with HUVECs treated with siNC. n = 3