Fig. 3

Angiogenic potential of EnSCs. a P3 HUVECs were suspended in CM from EnSC-Control or EnSC-EM-EC prepared as described in the “Materials and methods” section and were seeded into 96-well plates pre-coated with growth factor reduced Matrigel for 6 h. The tube-like structures were then imaged under an inverted microscope and analysed with ImageJ. b–f The total length, branch length, node number, branch number and junction number in the images of tube-like structures were quantitated with ImageJ. g P3 EnSC-Control or EnSC-EM-EC suspended in 200 μl of PBS were seeded into a silicone loop placed on top of the growing CAM between mature blood vessels and incubated for 3 days. Images were acquired in ovo under a stereomicroscope. h, i The vascular area and branch number in the images were observed and quantitated with ImageJ