Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The co-culture of ASCs and EPCs promotes vascularized bone regeneration in critical-sized bone defects of cranial bone in rats

Fig. 3

Analysis of results of co-culture of ASCs and EPCs in vitro. a Changes in ALP of ASCs and EPCs in different ratios (a ASCs, e EPCs). *p < 0.05. b RT-PCR detection of mRNA expression of osteogenic and angiogenic genes, osteogenic-related genes OCN, Col I, BMP2 and angiogenic-related genes VEGF, vWF, and cdh5 (a ASCs, e EPCs). *p < 0.05. c von Kossa staining. The number of calcified nodules in the ASC/EPC co-culture group was greater than that in the ASC and EPC groups. d The experimental results of Matrigel tubule formation assay under light microscope are presented. e Comparison of the number of tubular structures among the three groups, *p < 0.05. f Comparison of relative length of tubular structure among three groups, *p < 0.05. g The HA/Col void structure under scanning electron microscopy. h Cell growth morphology of ASCs/EPCs cocultured on HA/Col scaffolds in vitro

Back to article page