Fig. 8

Decreased ITGA3 and AKT protein expression in culture-aged TMSCs. a, b Changes in the expression of integrin family proteins induced by serial passaging were confirmed by Western blot analyses. c, d Changes in total and phosphorylated AKT protein expression between control (5 passage) and culture-aged TMSCs (25 passage) were also assessed. e Control TMSCs were transfected with either ITGA3 siRNA or control siRNA. b, d, f Bar graph represents relative changes in protein expression, determined by the intensity of each band normalized to that of the respective GAPDH band. The p values were considered statistically significant at the p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***), and significant differences among experimental groups were indicated with different alphabetical letters infigures