Fig. 3

The inhibition of TBI-induced apoptotic cell death by commercially available drugs. Mice were intraperitoneally administered the clinical dosage (10 μg/kg of body weight/day) of G-CSF once-daily for 4 times and RP once a week for 3 times (GRP #4) starting within 2 h after a 7.25-Gy dose of TBI. Mice treated with TBI only received injections of the normal saline solution as vehicle. “7.25 Gy + GRP” and “7.25 Gy” indicate the results of TBI mice with and without GRP #4, respectively. a Representative flow cytometry plots of Annexin V/PI stained populations in BMCs. b The number of Annexin V-positive PI-negative populations in the bone marrow (n = 5 in each group). c The number of Annexin V/PI double-positive populations in the bone marrow (n = 5 in each group). d Representative flow cytometry plots of Annexin V/PI stained populations in splenic cells (SPCs). e The number of Annexin V-positive PI-negative populations in the spleen (n = 5 in each group). f The number of Annexin V/PI double-positive populations in the spleen (n = 5 in each group). The data are expressed as the means ± SD. Statistically significant differences were evaluated by one-way ANOVA and the multiple comparison tests (**P < 0.01)