Fig. 5

The regulation of the expression of Nrf2 signaling pathway’s downstream genes. Mice were intraperitoneally administered a clinical dosage (10 μg/kg of body weight/day) of G-CSF once-daily for 4 times and RP once a week for 3 times (GRP #4) starting within 2 h after a 7.25-Gy dose of TBI. Mice treated with TBI only received injections of the normal saline solution as vehicle. “7.25 Gy” and “0 Gy” indicate the results of GRP #4 treated mice with and without TBI, respectively. The relative quantitative mRNA expression of aNqo1, Gclm, and Gsr in BMCs and bNqo1, Gclm, Fth1, and Txnrd1 in splenic cells, respectively, analyzed by qRT-PCR is presented as the means ± SD of the fold change (bar graph) compared with control mice (dotted line) (n = 5 in each group). ATP6 was used as an internal control for all reactions. Statistically significant differences were evaluated by one-way ANOVA and the multiple comparison tests (**P < 0.01 and *P < 0.05 against control, or ^††P < 0.01 and ^†P < 0.05 against drug combination mice without TBI)