Fig. 6
The effects of commercially available drugs on inflammation and cell senescence markers in serum. Mice were intraperitoneally administered a clinical dosage (10 μg/kg of body weight/day) of G-CSF once-daily for 4 times and RP once a week for 3 times (GRP #4) starting within 2 h after a 7.25-Gy dose of TBI. Mice treated with TBI only received injections of the normal saline solution as vehicle. “7.25 Gy + GRP” and “7.25 Gy” indicate the results of TBI mice with and without GRP #4, respectively. Peripheral blood was harvested from the orbital venous plexus of mice anesthetized using isoflurane, and serum samples were separated. The serum inflammation markers a PAI-1 and b TNF-α and the aging marker c CDKN2A/p16INK4a were analyzed by an ELISA (n = 5 in each group). The data are expressed as the means ± SD. Statistically significant differences were evaluated by one-way ANOVA and the multiple comparison tests (**P < 0.01)