Skip to main content

Table 1 Primers used for RT-PCR

From: Efficacy of matrilin-3-primed adipose-derived mesenchymal stem cell spheroids in a rabbit model of disc degeneration

Gene Primer sequence Accession number Amplicon (bp)
18S 5′-GTA ACC CGT TGA ACC CCA TT-3′
5′-CCA TCC AAT CGG TAG TAG CG-3′
NR_003286.2 151
SOX9 5′-GTA CCC GCA CTT GCA CAA C-3′
5′- TCT CGC TCT CGT TCA GAA GTC-3′
NM_000346.3 74
Collagen 2A 5′- GGGAGTAATGCAAGGACCA-3′
5′- ATCATCACCAGGCTTTCCAG-3′
NM_001844.4 175
Aggrecan 5′-GCC TGC GCT CCA ATG ACT-3′
5′-ATG GAA CAC GAT GCC TTT CAC-3′
NM_013227.3 104
Collagen A1 5′-CCC CTG GAA AGA ATG GAG ATG-3′
5′-TCC AAA CCA CTG AAA CCT CTG-3′
NM_000088.3 148
MMP13 5′-TCA CCA ATT CCT GGG AAG TCT-3′
5′-TCA GGA AAC CAG GTC TGG AG-3′
NM_002427.3 95
Collagen 10 5′-ACG CTG AAC GAT ACC AAA TG-3′
5′-TGC TAT ACC TTT ACT CTT TAT GGT GTA-3′
NM_000493.3 101
p53 5′-GGCCCACTTCACCGTACTAA-3′
5′-GTGGTTTCAAGGCCAGATGT-3′
NM_000546 156
BAX 5′-TTTGCTTCAGGGTTTCATCC-3′
5′-CAGTTGAAGTTGCCGTCAGA-3′
NM_001291428.1 246