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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages

Fig. 3

DNMT1 promotes HFSc differentiate into adipogenic lineages by inhibition of miR-214-3p. a The expression level of miR-214-3p was detected by RT-qPCR after the adipogenic differentiation of HFSc. b MSP detected the methylation level of miR-214-3p promoter after the adipogenic differentiation of HFSc. c ChIP detected the enrichment of DNMT1 on the miR-214-3p promoter after the HFScs were cultured in differentiation medium for 0 days, 7 days, and 14 days. d The expression level of miR-214-3p in each group was detected by RT-qPCR. e Western blot analysis was used to detect the expression level of DNMT1 normalized to GAPDH in each group. f The degree of adipogenic differentiation of HFSc was detected by oil red O staining. g Western blot analysis was used to detect the expression level of adipose formation markers normalized to GAPDH. The data were expressed as mean ± standard deviation. n = 3 per group. *p < 0.05

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