Fig. 4

miR-214-3p inhibits adipogenic differentiation of HFSc by downregulating MAPK1. a The binding sites between miR-214-3p and MAPK1 mRNA in 3′-UTR were predicted. b Western blot analysis was used to detect the expression of MAPK1 normalized to GAPDH in HFSc after adipogenic differentiation. c Dual-luciferase reporting experiment verified the targeting relationship between miR-214-3p and MAPK1. d Transfection efficiency of miR-214-3p after downregulating of miR-214-3p by RT-qPCR. e MAPK1 expression level normalized to GAPDH after inhibition of miR-214-3p was detected by Western blot analysis. f The expression levels of miR-214-3p in each group were detected by RT-qPCR. g Western blot analysis was used to detect the expression level of MAPK1 normalized to GAPDH in each group. h The degree of adipogenic differentiation of HFSc was detected by oil red O staining. i Western blot analysis was used to detect the expression level of adipose formation markers normalized to GAPDH. The data were expressed as mean ± standard deviation. n = 3 per group. *p < 0.05