Fig. 5

HUC-MSC treatment ameliorates IR through regulating the activation of ERK/MAPK signaling in the skeletal muscle of db/db mice. a Fixed skeletal muscle tissues were stained with ERK, and the intensity of immunohistochemical staining for ERK was evaluated by optical density. Representative images tissues from mice in each group. Magnification, × 400. b Skeletal muscle tissues were subjected to western blotting using specific antibodies against ERK and p-ERK. c mRNA expression levels of JNK, c-fos, and c-myc. Results are representative of 3 independent repeats. Data are presented as the mean ± standard deviation. *P < 0.05, **P < 0.01 vs. the baseline levels (db/m); ^#P < 0.05 and ^##P < 0.01