Fig. 4

Survival, neuronal maturation and mDA differentiation of the grafted cells. A Six months post-transplantation, immunofluorescence staining showed the survival (expression of hNuc) and the neuronal maturation (expression of NeuN) of the transplanted cells in one representative H-TH graft (upper panel). Portion of the grafted cells differentiated into dopaminergic neurons (FoxA2 and TH expression in the middle and lower panels). Dotted lines outline the graft. Scale bar, 500 μm. B Enlarged images showed portions of the grafted cells are mature neurons (NeuN expression), midbrain DA progenitors (FoxA2 expression) and DA neurons (TH expression) in high-TH and low-TH grafts respectively. Scale bar, 50 μm. C (a) quantification of the surviving transplanted cells in high-TH and low-TH grafts. (b) percentages of NeuN, FoxA2 and TH expressing cells within the high-TH and low-TH grafts (n = 3–5, *P < 0.05, 2-way ANOVA with post hoc Bonferroni test, data shown as mean ± SD)