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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: miR-150 regulates endothelial progenitor cell differentiation via Akt and promotes thrombus resolution

Fig. 1

Characterization of eEPCs and ECFCs. A Endogenous expression of miR-150 measured by real-time RT-PCR in eEPCs, ECFCs, and HUVECs. B Morphological changes of EPCs. (a) Peripheral blood mononuclear cells showed multiple morphologies immediately after plating. (b) Five days after seeding, PBMCs formed a central cluster. (c) On day 12, EPCs exhibited a spindle shape, endothelial cell-like morphology. (d) Two weeks after plating, ECFCs grown to confluence exhibited a cobblestone appearance similar to HUVEC. C ECFCs were stained with Dil-ac-LDL (red) and lectin (green) and the merged image showed double staining of Dil-ac-LDL and FITC-UEA-1 (yellow)

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