Fig. 3

miR-150 regulates the function of early EPCs and ECFCs. a miR-150 were regulated in eEPCs and ECFCs. Then, angiogenetic capacity was examined in eEPCs, eEPCs transfected with miR-150 agomir or antagomir, ECFCs, and ECFCs transfected with miR-150 agomir or antagomir. Representative images (upper) and statistical analysis of three independent experiments (lower) are shown. b eEPCs transfected with miR-150 agomir or antagomir and ECFCs transfected with miR-150 agomir or antagomir were mixed with Matrigel and then injected subcutaneously on nude mice. The gel plug was removed after 1 week for the analysis of angiogenetic intensity. c The gel plugs were texted with HE stain (× 200). d CCK-8 assay was conducted to test the proliferation capacity in different groups (eEPCs, eEPCs+miR-150 agomir, eEPCs+miR-150 agomir, ECFCs and ECFCs+miR-150 agomir, ECFCs+miR-150 antagomir). e Concentrations of IL-8 and VEGF were measured in supernatant among different groups (eEPCs, eEPCs+miR-150 agomir, eEPCs+miR-150 agomir, ECFCs and ECFCs+miR-150 agomir, ECFCs+miR-150 antagomir). (*P < 0.05 vs. eEPCs; **P < 0.01 vs. eEPCs; ^#P < 0.05 vs. ECFCs)