Fig. 5

Effect of miR-150 during EPC differentiation is mediated by targeting c-Myb. a c-Myb protein level in eEPCs and ECFCs, measured by Western blot. b Dual-luciferase reporter assay was performed on 293T cells. Schematic graph of the constructed reporter plasmid containing putative binding sites of miR-150 in the c-Myb 3′UTR. c-Myb 3′UTR mut indicates mutation in the miR-150 binding site (upper). Relative luciferase activity of c-Myb 3′UTR decreased as compared to mutation control (lower). c The effect of miR-150 on c-Myb expression was confirmed by real-time RT-PCR (left) and Western blot (right). d Western blot analysis revealed the effect of miR-150 on the Akt/FOXO1 pathway