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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Dose-effect relationship and molecular mechanism by which BMSC-derived exosomes promote peripheral nerve regeneration after crush injury

Fig. 1

Characterization of BMSC-derived EXOs and cell viability. EXOs from the BMSC culture supernatant were assessed by TEM, NTA, and flow cytometry. a Protocol for EXO isolation. b Ultracentrifuged components of BMSC culture medium as observed by TEM. Arrows indicate particles of EXOs. c A schematic diagram showing the distribution of the average diameter of EXOs observed under TEM. d BMSC culture medium was subjected to ultra-high-speed centrifugation and detected by NTA. e Flow cytometry profiles of paired CD44 and CD45 marker proteins. Scale bar = 100 nm in b. f Viability measurements of BMSCs after a 72-h culture in 10% FBS and serum-free conditions. The values were normalized to present the control (10% FBS) as 100% cell viability

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Stem Cell Research & Therapy

ISSN: 1757-6512