Fig. 4

Regenerated nerve fibers were counted on the 28th day after SNC. On day 28 after surgery, the distal regenerated nerve trunk was harvested from the crush point, and transverse sections were subjected to toluidine blue staining. Regenerated fibers were observed under a microscope, and the number and diameter of regenerated myelinated fibers were statistically analyzed. a Schematic of the experimental design for sciatic nerve injury and functional recovery. b List of groups in the gastrocnemius injection experiments and the number of exosome particles injected. c, d Images at 100-fold or 400-fold magnification, respectively. The area in the large box was enlarged in the small box, which shows the shape of the regenerated myelinated fibers in d. Scale bar = 100 μm in c, 20 μm in d. e Statistical analysis of the number of regenerated myelinated fibers. f Statistical analysis of the diameters of regenerated myelinated nerve fibers. Asterisks indicate that the difference between the two groups was statistically significant. Three asterisks indicate p < 0.001, two asterisks indicate p < 0.01, and a single asterisk indicates p < 0.05. Number sign indicates that compared with the PBS group, p < 0.01, the difference was statistically significant