Fig. 4

SIRT1-modified hUCMSCs attenuated EMT in PD-induced peritoneal damage. a Immunohistochemical analyses of α-SMA expression in peritoneal tissues (bar 200 μm). b Accumulation of α-SMA-positive area of a. qRT-PCR was used to measure the mRNA levels of α-SMA (c), Fibronectin (d), Col III (e), Snail (f), and Calretinin (g) in the peritoneal omentum tissues from the indicated groups. h Western blot was used to measure the protein levels of Fibronectin, α-SMA, Snail, and Calretinin in the indicated conditions, and the relative expressions were normalized to control (i). Data are presented as mean ± SD. n = 8 for each group. *p < 0.05, **p < 0.01, and ***p < 0.001 between the indicated groups