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Table 2 Analysis of cell surface marker expressions by flow cytometry after transfection (%, mean ± SD)

From: SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway

Groups (hUCMSCs) Number CD105 CD90 CD34 CD45
Control 4 97.86 ± 1.23 98.05 ± 0.89 1.21 ± 0.32 1.98 ± 0.43
Ad-NC 4 96.76 ± 1.62 97.03 ± 2.62 0.96 ± 0.21 1.38 ± 0.39
Ad-SIRT1 4 97.15 ± 0.92 96.61 ± 1.62 1.31 ± 0.62 2.23 ± 0.85
p value 0.324 0.186 0.294 0.106
  1. hUCMSCs among different groups were analyzed for CD 105, 90, 34, and 45 expressions by using fluorescence-activated cell sorting (FACS) with flow cytometry at passage 6. The negative expressions of CD 34 and 45 and positive expressions of CD 105 and 90 indicated a remaining mesenchymal stem cell lineage after Ad-SIRT1 transfection