Skip to main content
Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells

Fig. 2

Generation of engineered human umbilical arterial endothelial cells by transduction of adenoviral E4orf1 and GFP (HuAECs-E4orf1-GFP). a Schematic illustration of the protocol for test our hypothesis on the role of HuAECs in supporting ex vivo HSPC expansion. b Fluorescence intensity of GFP in HuAECs-E4orf1-GFP and HuVECs-E4orf1-GFP before FACS (above panel); the image of GFP expression in HuAECs-E4orf1-GFP and HuVECs-E4orf1-GFP after FACS (button panel). c Detection of E4ORF1 expression in HuAECs-E4orf1-GFP and HuVECs-E4orf1-GFP by qRT-PCR. d qRT-PCR analysis of arterial and venous markers expression in HuAECs-E4orf1-GFP and HuVECs-E4orf1-GFP. e Comparison of the relative expression of ANGPTL4, IGF2, and HOXB4 in HuAECs-E4orf1-GFP and HuVECs-E4orf1-GFP. *P < 0.05, **P < 0.01 ***P < 0.001; n = 3, scale bar, 200 μm

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512