Fig. 3

CD34⁺ hCB cells cocultured with HuAECs-E4orf1-GFP resulted in a significant expansion of HSPCs while heightened in vitro multilineage differentiation potential. a The cumulative curve of TNCs during the expansion. b TNC expansion. c Total CD34⁺ hematopoietic cell expansion. d Representative scatter plots of CD34⁺CD38⁻CD90⁺ cell expansion. e The number of CD34⁺CD38⁻ cells (left panel) and CD34⁺CD38⁻CD90⁺ cells (right panel) significantly increased in the HuAECs-E4orf1-GFP group at day 14. f CFU numbers of amplified CD34⁺ hCB cells in different groups after 14-day expansion. g CFU (CFU-E, BFU-E, CFU-GM, CFU-M, and CFU-G) morphology (above panel, scale bar: 500 μm) and Giemsa’s staining for cytospin samples of CFUs (bottom panel, scale bar: 100 μm). *P < 0.05, **P < 0.01, ***P < 0.001; n = 8. Three HuAECs-E4orf1-GFP and HuVECs-E4orf1-GFP cell lines were established and all of them have stable and consistent effects on ex vivo expansion of HSPCs