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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells

Fig. 4

CD34+ hCB cells cocultured with HuAECs-E4orf1-GFP have repopulating capability in vivo. a Schematic illustration of the protocol for in vivo transplantation. NSG mice were transplanted with 1,000,000 cells from each culture after sublethally irradiation. b Representative scatter plots of human CD45+ hematopoietic cell engraftment in mouse’s spleen after 16-week transplantation (left panel). Chimerism of engrafted human CD45+ hematopoietic cells in mouse’s spleen after 16-week transplantation (right panel). c Representative scatter plots of human CD45+ hematopoietic cell engraftment in mouse’s BM after 16-week transplantation (left panel). Chimerism of engrafted human CD45+ hematopoietic cells in mouse’s BM after 16-week transplantation (right panel). Formula: chimerism (%) = [%hCD45+/(%hCD45++%mCD45+)]. *P < 0.05, **P < 0.01; n = 8

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