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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Arterial endothelium creates a permissive niche for expansion of human cord blood hematopoietic stem and progenitor cells

Fig. 5

CD34+ hCB cells amplified on HuAECs-E4orf1-GFP exhibited Notch signaling activation. a The CD34+ hCB cell expansion at day 14 and CFU formation in CpE treatment group or with a vehicle control (DMSO) group. b Image of GFP expression in HuAECs-E4orf1-shLV6 (left panel) and HuAECs-E4orf1-shDLL4 (right panel) after FACS. c Expression of DLL4 in HuAECs-E4orf1-shLV6 and HuAECs-E4orf1-shDLL4. d The number of hematopoietic cells and CFU decreased in the HuAECs-E4orf1-shDLL4 group. e Hypothesis for the signal pathways involved in DLL4-mediated promoting proliferation activity of HuAECs-E4orf1 was indicated. NS, no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001; n = 8; scale bar, 200 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512