Table 1 Quantitative RT-PCR primer sequences
Gene | Sequence (5′-3′) |
|---|---|
CXCR4 | F:GCCTTATCCTGCCTGGTATTGTC |
R:GCGAAGAAAGCCAGGATGAGGAT | |
DLL1 | F:GACGAACACTACTACGGAGAGG |
R:AGCCAGGGTTGCACACTTT | |
DLL4 | F:TGGGTCAGAACTGGTTATTGGA |
R:GTCATTGCGCTTCTTGCACAG | |
EFNB2 | F:TTCAGCCCTAACCTCTGGGG |
R:CCTCCAAAGACCCATTTGATGTA | |
GJA4 | F:TGCAAGAGTGTGCTAGAGGC |
R:ACAAAGCAGTCCACGAGGTAG | |
HEY1 | F:GTTCGGCTCTAGGTTCCATGT |
R:CGTCGGCGCTTCTCAATTATTC | |
HEY2 | F:CCTAACAGAAGTTGCGCGGTA |
R:GAGGCGACAAGGGGTTGAC | |
JAG1 | F:GTCCATGCAGAACGTGAACG |
R:GCGGGACTGATACTCCTTGA | |
NOTCH1 | F:GAGGCGTGGCAGACTATGC |
R:CTTGTACTCCGTCAGCGTGA | |
NOTCH2 | F:CAACCGCAATGGAGGCTATG |
R:GCGAAGGCACAATCATCAATGTT | |
NOTCH3 | F:CGTGGCTACACTGGACCTC |
R:AGATACAGGTGAACTGGCCTAT | |
NOTCH4 | F:TGTGAACGTGATGTCAACGAG |
R:ACAGTCTGGGCCTATGAAACC | |
APLNR | F:CCTGCATCAGCTACGTCAACA |
R:GGGATGGATTTCTCGTGCATCT | |
EPHB4 | F:CGCACCTACGAAGTGTGTGA |
R:GTCCGCATCGCTCTCATAGTA | |
NR2F2 | F:AACCAGCCGACGAGATTCG |
R:CCCGGATGAGGGTTTCGATG | |
NRP2 | F:CCAACGGGACCATCGAATCTC |
R:CCAGCCAATCGTACTTGCAGT | |
E4ORF1 | F:CGCCGGAATTAGATCTGCCA |
R:CTCGAGCAGCGTAATCTGGA | |
ANGPTL4 | F:GGCTCAGTGGACTTCAACCG |
R:CCGTGATGCTATGCACCTTCT | |
IGF2 | F:GTGGCATCGTTGAGGAGTG |
R:CACGTCCCTCTCGGACTTG | |
HOXB4 | F:GCACGGTAAACCCCAATTA |
R:GGCAACTTGTGGTCTTTTTT | |
GATA2 | F:GCAACCCCTACTATGCCAACC |
R:CAGTGGCGTCTTGGAGAAG | |
RUNX1 | F:ATGTGGTCCTATTTAAGCCAGCCC |
R:TCATCTGGCTGAAGACACCAGCTT | |
HES1 | F:TCAACACGACACCGGATAAAC |
R:GCCGCGAGCTATCTTTCTTCA | |
GAPDH | F:GAGTCAACGGATTTGGTCGT |
R:TTGATTTTGGAGGGATCTCG |