Fig. 5

Knocking down tsRNA-16902 leads to impaired the adipogenic differentiation of these hMSCs after 0, 7, and 14 days. a hMSCs subjected to oil red O staining were assessed via light microscope at different time points (20×); scale bar, 20 μm. b A microplate reader was used in order to quantify oil Red O staining intensity. c PPARγ, FABP4, C/EBPα, and RARγ expression was analyzed using qRT-PCR. d PPARγ, FABP4, C/EBPα, and RARγ expression was analyzed via Western blotting. Data are means ± SD (n = 3). **P < 0.01 vs. negative controls, respectively. Note: NC, negative control; sh, tsRNA-16902 shRNA