Fig. 8

Knockdown of tsRNA-16902 or overexpression of RARγ leads to increased p-Smad2/3 levels, and addition of a Smad2/3 inhibitor reduced p-Smad2/3 levels in the context of adipogenesis as determined by measuring Smad2/3 phosphorylation by Western blotting on days 0, 7 and 14. a shRNA-mediated knockdown of tsRNA-16902 alters p-Smad2/3 levels in the context of adipogenic differentiation. b RARγ overexpression alters p-Smad2/3 levels in the context of adipogenic differentiation. c Smad2/3 inhibition reduced p-Smad2/3 levels during the adipogenic differentiation of hMSCs. Data are expressed as means ± SD (X ± SD, n = 3). **P < 0.01 vs. negative controls, respectively (a, b); **P < 0.01 vs. tsRNA-16902 shRNA + inhibitor (c). Note: sh, shRNA; sh+inh, sh + inhibitor. Note: NC, negative control; sh, tsRNA-16902 shRNA; OE, overexpression RARγ; sh+in, tsRNA-16902 shRNA + inhibitor; p-Smad2/3, Smad2/3 phosphorylation; T-Smad2/3, total Smad2/3