Fig. 2

Aspirin suppresses cancer cell metastasis and stemness in vivo. a Schema of the metastasis model established by subcutaneous implantation of 4T1-luci cells into the 4th pair of mammary fat pad of BALB/c mice. b Tumor growth curve of 4T1-luci with or without aspirin treatment. c The body weight of BALB/c mice in the course of aspirin treatment. d The survival curve of BALB/c mice inoculated with 4T1-luci with or without aspirin treatment. e The representative luciferase images showing the 4T1-luci tumors at the primary site and lung metastasis sites with or without aspirin treatment. f Representative H&E staining images of 4T1-luci tumors metastasis to the lung with or without aspirin treatment. Scale bars (lower panel), 100 μm. g Statistic results of metastasis loci of 4T1-luci tumors metastasis to the lung with or without aspirin treatment. h Immunohistochemistry staining of SOX2 and OCT4 in 4T1-luci primary tumors with or without aspirin treatment. Representative images with × 20 magnification were shown. Scale bars, 100 μm. i Statistic results of SOX2 or OCT4 positive cells in 4T1-luci primary tumors with or without aspirin treatment. j Schema of the chemo-resistance model established by subcutaneous implantation of 4T1-luci cells into the 4th pair of mammary fat pad of BALB/c mice. k Tumor growth curve of 4T1-luci with or without aspirin treatment in the presence of cisplatin. l The body weight of BALB/c mice in the course of aspirin treatment in the presence of cisplatin. m The survival curve of BALB/c mice inoculated with 4T1-luci with or without aspirin treatment in the presence of cisplatin. n The representative luciferase images showing 4T1-luci tumors at the primary sites with or without aspirin treatment on day 7 (before cisplatin administration) and day 18 (after cisplatin administration). o Immunohistochemistry staining of SOX2 and OCT4 in 4T1-luci primary tumors with or without aspirin treatment in the presence of cisplatin. Representative images with × 20 magnification were shown. Scale bars, 100 μm. p Statistic results of SOX2 or OCT4 positive cells in 4T1-luci primary tumors with or without aspirin treatment in the presence of cisplatin