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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Aspirin mediates histone methylation that inhibits inflammation-related stemness gene expression to diminish cancer stemness via COX-independent manner

Fig. 3

Aspirin inhibits the expression of inflammation-related stemness genes in vitro and in vivo. a Schematic representation of the siRNA screen (left). Summary of the results from the RNAi screen (right). b Western blot examining the expression of inflammatory candidates and stemness proteins (SOX2, OCT4) in A549, MDA-MB-231, and HepG2 cells with or without aspirin treatment. c Quantitative PCR examining the mRNA expression of inflammatory candidates and stemness genes (SOX2, OCT4) in A549, MDA-MB-231, and HepG2 cells with or without aspirin treatment. d Immunofluorescence staining of inflammatory candidates and stemness genes (SOX2, OCT4) in A549, MDA-MB-231, and HepG2 cells with or without aspirin treatment. Scale bars, 10 μm. e Quantitative PCR examining the mRNA expression of inflammatory candidates and stemness genes (SOX2, OCT4) in 4T1-luci tumors separated from BALB/c mice treated with aspirin for different survival days. f Western blot examining the expression of inflammatory candidates and stemness genes (SOX2, OCT4) in 4T1-luci tumors separated from BALB/c mice treated with aspirin for different survival days

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