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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Aspirin mediates histone methylation that inhibits inflammation-related stemness gene expression to diminish cancer stemness via COX-independent manner

Fig. 4

Aspirin mediates histone 3 methylation to effect target genes expression in vitro and in vivo. a Western blot examining the expression of histone 3 methylation markers, KDM6A, KDM6B in A549, MDA-MB-231, and HepG2 cells with or without aspirin treatment. b Immunofluorescence staining of H3 methylation markers in A549, MDA-MB-231, and HepG2 cells with or without aspirin treatment. Scale bars, 10 μm. c Western blot to check the expression of H3-3Me markers in nuclear proteins of each groups. d CHIP-qPCR detecting the expression of ICAM3 DNA fragment in A549, MDA-MB-231, and HepG2 cells with or without aspirin treatment. e Immunohistochemistry staining examining the expression of H3 methylation markers in 4T1-luci tumors separated from BALB/c mice. Representative images with × 40 magnification were shown. Scale bars, 50 μm. f The bar graph shows the statistic results of H3 methylation markers positive cells. g CHIP-qPCR detecting the expression of ICAM3 DNA fragment in 4T1-luci tumors separated from BALB/c mice with or without aspirin treatment

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Stem Cell Research & Therapy

ISSN: 1757-6512