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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Aspirin mediates histone methylation that inhibits inflammation-related stemness gene expression to diminish cancer stemness via COX-independent manner

Fig. 5

Aspirin mediates H3 methylation to regulate ICAM3 expression via a COX-independent manner. a Western blot examining the COX1 and COX2 knockdown efficiency in A549 cells. b ALDH staining assay was performed to check ALDH+ sub-population percentage in A549-shCtrl, shCOX cells with or without aspirin treatment. c Side population assay was performed to detect SP percentage in A549-shCtrl, shCOX cells with or without aspirin treatment. d FACS was performed to detect cell resistance to cisplatin, and the percentage of apoptotic cells was shown. e–g Statistic results of ALDH+ sub-population, side population, and apoptosis cell percentage were shown. h Western blot examining the expression of histone 3 methylation markers, KDM6A, KDM6B in A549-shCtrl, shCOX cells with or without aspirin treatment. i Western blot examining the expression of ICAM3 in A549-shCtrl, shCOX cells with or without aspirin treatment

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Stem Cell Research & Therapy

ISSN: 1757-6512