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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus

Fig. 1

Flowchart of the study design. Stabilized femur fractures were established in 40 SD rats. Radiographs and micro-CT analysis examined the effect of Short-Wave on fracture healing. The expression of HIF-1 and other factors in callus was tested by q RT-PCR. SDF-1 in plasma was evaluated by ELISA. To analyze the MSC migration in healing, in vivo fluorescence assays and immunofluorescence were used after treatment in 20 nude mice with a femoral fracture. For in vitro study, osteoblast simulated fracture site was first irradiated by the Short-Wave; CoCl2 in medium stimulated hypoxia condition; siRNA targeting HIF-1 was used to investigate the role of HIF-1. Osteoblast culture medium was then collected as chemotaxis content of MSC, and the migration of MSC was evaluated using wound healing assay and trans-well chamber test. The expression of HIF-1 and its related factors were quantified by q RT-PCR, ELISA, and Western blot. SW Short-Wave treatment, MG GFP-labeled MSC. The image element in the flowchart mainly comes from https://app.biorender.com/

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