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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Combined therapy with adipose tissue-derived mesenchymal stromal cells and meglumine antimoniate controls lesion development and parasite load in murine cutaneous leishmaniasis caused by Leishmania amazonensis

Fig. 4

Analysis of cytokine concentrations in cell culture supernatants. Concentrations of the cytokines TNF-α (a), IL-10 (b), and VEGF (c) present in supernatants of cell cultures were determined by enzyme-linked immunosorbent assay (ELISA) using commercial BD kits, according to the manufacturer’s instructions. Groups: Macrophages (Mɸ); macrophages co-cultured with BM-MSCs (Mɸ + BM-MSC); macrophages co-cultured with AD-MSCs (Mɸ + AD-MSC); macrophages infected with Leishmania amazonensis (Mɸ + La); macrophages infected with Leishmania amazonensis co-cultured with BM-MSCs (Mɸ + La + BM-MSC); macrophages infected with Leishmania amazonensis co-cultured with AD-MSCs (Mɸ + La + AD-MSC). Representative of two independent experiments. N = 3. Values show the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001 indicate a significant difference between the groups. ND not detected

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Stem Cell Research & Therapy

ISSN: 1757-6512