Fig. 6
Lymph node cell count and detection of T CD4+ and Treg lymphocytes by flow cytometry (FACS). Cells were collected from the draining popliteal lymph node macerate, counted under a microscope (× 4 magnification), and analyzed by flow cytometry (FACS CANTO BD) for T CD4+ and Treg (CD4+ CD25+ FoxP3+) lymphocyte expression after 52 days of infection. Results shown as percentage and total population of cells positive for these markers in CD3+ marker-positive lymphocytes. a Total number of lymph node cells. b Percentage of CD4+ T lymphocytes. c Total CD4+ T lymphocyte population. d Percentage of Treg lymphocytes. e Treg lymphocyte population. Values show the mean ± standard deviation for each group. *P < 0.05 **, P < 0.01, and ***P < 0.005 indicate a significant difference between the groups in relation to the control (PBS); #P < 0.05, ##P < 0.01, and ###P < 0.005 indicate a significant difference between the groups in relation to the AD-MSC; +P < 0.05 indicates a significant difference in the AD-MSC group in relation to the PA group