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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Vagal α7nAChR signaling regulates α7nAChR+Sca1+ cells during lung injury repair

Fig. 1

Flow cytometric and immunofluorescent analysis of the bone marrow α7nAChR-expressing progenitor cells. a Flow cytometric analysis of the bone marrow α7nAChR-, CD34-, Flk1-, CXCR4-, and Sca1-expressing cells. The bone marrow mononuclear cells were isolated from normal mice and pneumonia mice at 3 days after intratracheal E. coli challenge and stained with anti-α7nAChR, CD34, Flk1, CXCR4, and Sca1 fluorescent antibodies. Cells were analyzed in the lymphocyte gate. Representative results from individual mice are shown. b Dynamic changes in extravascular lung water (ELW) in E. coli pneumonia. Mice were intratracheally challenged with E. coli (2.5 × 106 CFU) and sacrificed for 7 consecutive days. The lungs were collected to measure ELW, an index of pulmonary inflammation and edema. N = 3–5 in each group. Two-way ANOVA was used. *P < 0.05 versus normal control. c Immunofluorescent detection of BM α7nAChR+Sca1+ cells. BM-MNCs isolated from normal and pneumonia mice at 3 dpi. The cells were subjected to immunofluorescent staining. d Flow cytometry analysis of dynamic changes in BM α7nAChR+Sca1+ cells. Mice were intratracheally challenged with E. coli (2.5 × 106 CFU) and sacrificed for 7 consecutive days. BM cells were isolated, stained with anti-α7nAChR and Sca1 fluorescent antibodies, and subjected to flow cytometry. Normal BM cells were used as controls. N = 3–5 in each group. Two-way ANOVA was used. *P < 0.05 versus normal control. Data are shown as the mean ± SD

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