Fig. 1

Differentiation of hES2 and hiPS into SSCLCs by a three-step approach. a Schematic illustration of a three-step protocol to differentiate hES2 and hiPS into SSCLCs. TTE, testosterone; RA, retinoic acid. b Cell morphology of differentiated cells derived from hES2 and hiPS at stage 1–stage 3 (S1-S3). Scale bars, 50 μm. Black arrows indicate the cells with grape-like phenotype. Bright arrows indicate the cells with fibroblastic phenotype. c Phase-contrast images and immunostaining images of the differentiated cells at stage 3 with SSC marker antibodies against UCHL1, CD90, GPR125, VASA, GFRA1, PLZF, MAGEA4, and PCNA. Mouse IgG/corresponding Alexa Fluor 594, and Rabbit IgG/corresponding Alexa Fluor 488 were used as negative control. Scale bars, 50 μm. d Quantification of the percentages of UCHL1 and CD90 double-positive cells over the total cells. e Immunostaining images of the differentiated cells derived from hiPS at stage 1 and stage 2 with the antibodies against UCHL1 and CD90. Scale bars, 50 μm