Fig. 2

hES2-SSCLCs and hiPS-SSCLCs were maintained in vitro for a long time. a Phase-contrast images of the cell morphology of SSCLCs at P1-P7. Black arrows indicate grape-like morphology of SSCLCs. Bright arrows indicate fibroblast-like cells (FBs). Scale bars, 50 μm. b Immunostaining images of SSCLCs clusters derived from hES2 and hiPSC at passage 2 with the antibodies against CD90, GFRA1, GPR125, PLZF, and VASA. Rabbit/mouse IgG and corresponding Alexa Fluor 594 were used as negative control. Scale bars, 50 μm. c Cell number of VASA and UCHL1 double-positive cells at the indicated time points and passages. Cell counts are mean ± SEM from three independent different experiments. d Immunostaining images of SSCLCs at P7 with the antibodies against VASA, UCHL1, CD90, GFRA1, PLZF, and GPR125. Scale bars, 50 μm