Fig. 3

Meiotic procession of SSCLCs and formation of haploid cells. a Meiotic spread assays of differentiated cells derived from SSCLCs were performed with the antibodies against SCP3, MHL1, and CREST. hiPS were used as negative control. Scale bars, 10 μm. b Haploid cells derived from hES2-SSCLCs and hiPS-SSCLCs at P2 were analyzed by FACS with Hoechst 33342. The human testicular cells derived from OA patients were used as positive control. hiPS were used as negative control. c Quantification of the percentages of haploid cells in different group. d FACS-isolated haploid cells were stained with acrosin and DAPI. Scale bars, 50 μm in low magnification images. Scale bars, 5 μm in high magnification. Rabbit IgG and corresponding Alexa Fluor 594 were used as negative control. e FISH analyses of sex chromosomes and Chr 18 on diploid cells and haploid cells derived from SSCLCs. f A quantification of haploid cells of FISH signal of X chr or Y chr. The rate represents the number of haploid cells over total haploid cells isolate by FACS. At least 100 cells were counted per experiment and at least three independent experiments were performed. Data are represented as mean ± SEM