Fig. 4

Comparison of the transcriptomes of hPSCs, SSCLCs, and enriched GPR125⁺ cells isolated from human testes. a, b Characterization of enriched GPR125⁺ Cells isolated from human testes of OA patients or SSCLCs. Phase contrast and identification of immunoassay of GPR125⁺ cells isolated from human testes of OA patients or hiPS-SSCLCs with antibodies against GPR125, CD90, UCHL1, VASA, GFRA1, and PLZF. a Enriched GPR125⁺ cells from human testes of OA patients. b hiPS-SSCLCs. Scale bars, 50 μm. c Heatmap and hierarchical clustering of significant genes expression of 1042 genes (P < 0.001) in SSCLCs at P1 or P7 and enriched GPR125⁺cells isolated from human testes. SLC represents SSCLCs. SSC1, SSC2, and SSC3 represent enriched GPR125⁺cells isolated from three OA patients. Data are represented as mean ± SEM. See also Table S2. d Heatmap on transcript expression of SSC markers, germ cell-related genes in hPSCs, SSCLCs, and enriched GPR125+ cells isolated from human testes. See also Table S3. e GO biological enrichment analysis of differential genes whose expression changes ≥ 2-fold identified between enriched GPR125⁺ cells and SSCLCs. BP represents biological process. Data are represented as mean ± SEM. Also see Table S2